Construct: shRNA TRCN0000190563

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028814.1-2763s1c1
DNA Barcode:
GCGTCTGAGATACTGTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2810403A07Rik (74200)
Gene Description:
RIKEN cDNA 2810403A07 gene
Transcript:
RefSeq NM_028814.1 (NON-CURRENT)
Match location:
Position 2763 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190563)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTCTGAGATACTGTCATTT
Hairpin Sequence:
5'-CCGG-GCGTCTGAGATACTGTCATTT-CTCGAG-AAATGACAGTATCTCAGACGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTCTGAGATACTGTCATTTCTCGAGAAATGACAGTATCTCAGACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTCTGAGATACTGTCATTTCTCGAGAAATGACAGTATCTCAGACGC-3'

Other clones with same target sequence:

(none)