Construct: shRNA TRCN0000190580

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019424.1-2451s1c1
DNA Barcode:
GCAGAAGAACGTCTTAGTGCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hps1 (192236)
Gene Description:
HPS1, biogenesis of lysosomal organelles complex 3 subunit 1
Transcript:
RefSeq NM_019424.1 (NON-CURRENT)
Match location:
Position 2451 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190580)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAGAACGTCTTAGTGCT
Hairpin Sequence:
5'-CCGG-GCAGAAGAACGTCTTAGTGCT-CTCGAG-AGCACTAAGACGTTCTTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAGAACGTCTTAGTGCTCTCGAGAGCACTAAGACGTTCTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAGAACGTCTTAGTGCTCTCGAGAGCACTAAGACGTTCTTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000292554