Construct: shRNA TRCN0000190628

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153585.3-1255s1c1
DNA Barcode:
GCCTTCGAGTGTCTGATTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cnot10 (78893)
Gene Description:
CCR4-NOT transcription complex, subunit 10
Transcript:
RefSeq NM_153585.3 (NON-CURRENT)
Match location:
Position 1255 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190628)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCGAGTGTCTGATTGAA
Hairpin Sequence:
5'-CCGG-GCCTTCGAGTGTCTGATTGAA-CTCGAG-TTCAATCAGACACTCGAAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCGAGTGTCTGATTGAACTCGAGTTCAATCAGACACTCGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCGAGTGTCTGATTGAACTCGAGTTCAATCAGACACTCGAAGGC-3'

Other clones with same target sequence:

(none)