Construct: shRNA TRCN0000190651

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025336.1-926s1c1
DNA Barcode:
GCCATGTTCTAGATCAGCGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chchd3 (66075)
Gene Description:
coiled-coil-helix-coiled-coil-helix domain containing 3
Transcript:
RefSeq NM_025336.1 (CURRENT)
Match location:
Position 926 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190651)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGTTCTAGATCAGCGAT
Hairpin Sequence:
5'-CCGG-GCCATGTTCTAGATCAGCGAT-CTCGAG-ATCGCTGATCTAGAACATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGTTCTAGATCAGCGATCTCGAGATCGCTGATCTAGAACATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGTTCTAGATCAGCGATCTCGAGATCGCTGATCTAGAACATGGC-3'

Other clones with same target sequence:

(none)