Construct: shRNA TRCN0000190709

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177791.2-1190s1c1
DNA Barcode:
GCCAATGAAAGTGGCTCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kctd19 (279499)
Gene Description:
potassium channel tetramerisation domain containing 19
Transcript:
RefSeq NM_177791.2 (NON-CURRENT)
Match location:
Position 1190 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190709)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATGAAAGTGGCTCTGAA
Hairpin Sequence:
5'-CCGG-GCCAATGAAAGTGGCTCTGAA-CTCGAG-TTCAGAGCCACTTTCATTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATGAAAGTGGCTCTGAACTCGAGTTCAGAGCCACTTTCATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATGAAAGTGGCTCTGAACTCGAGTTCAGAGCCACTTTCATTGGC-3'

Other clones with same target sequence:

(none)