Construct: shRNA TRCN0000190732

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178663.2-1250s1c1
DNA Barcode:
GCCAGAAGAAGGTTGAAACGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bend7 (209645)
Gene Description:
BEN domain containing 7
Transcript:
RefSeq NM_178663.2 (NON-CURRENT)
Match location:
Position 1250 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190732)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGAAGAAGGTTGAAACGA
Hairpin Sequence:
5'-CCGG-GCCAGAAGAAGGTTGAAACGA-CTCGAG-TCGTTTCAACCTTCTTCTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGAAGAAGGTTGAAACGACTCGAGTCGTTTCAACCTTCTTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGAAGAAGGTTGAAACGACTCGAGTCGTTTCAACCTTCTTCTGGC-3'

Other clones with same target sequence:

(none)