Construct: shRNA TRCN0000190782

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_206537.1-814s1c1
DNA Barcode:
GCCCAGGAAGATGACAATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp2c54 (404195)
Gene Description:
cytochrome P450, family 2, subfamily c, polypeptide 54
Transcript:
RefSeq NM_206537.1 (NON-CURRENT)
Match location:
Position 814 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190782)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGGAAGATGACAATCAT
Hairpin Sequence:
5'-CCGG-GCCCAGGAAGATGACAATCAT-CTCGAG-ATGATTGTCATCTTCCTGGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGGAAGATGACAATCATCTCGAGATGATTGTCATCTTCCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGGAAGATGACAATCATCTCGAGATGATTGTCATCTTCCTGGGC-3'

Other clones with same target sequence:

(none)