Construct: shRNA TRCN0000190820
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_029815.1-2486s1c1
- DNA Barcode:
- GCTGTAAAGAAGAATGGGCAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Bcas1 (76960)
- Gene Description:
- breast carcinoma amplified sequence 1
- Transcript:
- RefSeq NM_029815.1 (NON-CURRENT)
- Match location:
- Position 2486 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000190820)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTGTAAAGAAGAATGGGCAA
- Hairpin Sequence:
-
5'-CCGG-GCTGTAAAGAAGAATGGGCAA-CTCGAG-TTGCCCATTCTTCTTTACAGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTGTAAAGAAGAATGGGCAACTCGAGTTGCCCATTCTTCTTTACAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTGTAAAGAAGAATGGGCAACTCGAGTTGCCCATTCTTCTTTACAGC-3'
Other clones with same target sequence:
(none)
