Construct: shRNA TRCN0000190826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177190.2-1130s1c1
DNA Barcode:
GCCTACCTGAAGCTTCTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tcp11l1 (320554)
Gene Description:
t-complex 11 like 1
Transcript:
RefSeq NM_177190.2 (NON-CURRENT)
Match location:
Position 1130 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTACCTGAAGCTTCTGAAA
Hairpin Sequence:
5'-CCGG-GCCTACCTGAAGCTTCTGAAA-CTCGAG-TTTCAGAAGCTTCAGGTAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTACCTGAAGCTTCTGAAACTCGAGTTTCAGAAGCTTCAGGTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTACCTGAAGCTTCTGAAACTCGAGTTTCAGAAGCTTCAGGTAGGC-3'

Other clones with same target sequence:

(none)