Construct: shRNA TRCN0000190865

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172963.3-240s1c1
DNA Barcode:
GCAGAAGTATAAGTCCCTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtcl1 (68617)
Gene Description:
microtubule crosslinking factor 1
Transcript:
RefSeq NM_172963.3 (NON-CURRENT)
Match location:
Position 240 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190865)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAGTATAAGTCCCTCTA
Hairpin Sequence:
5'-CCGG-GCAGAAGTATAAGTCCCTCTA-CTCGAG-TAGAGGGACTTATACTTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAGTATAAGTCCCTCTACTCGAGTAGAGGGACTTATACTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAGTATAAGTCCCTCTACTCGAGTAGAGGGACTTATACTTCTGC-3'

Other clones with same target sequence:

(none)