Construct: shRNA TRCN0000190964

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178605.2-913s1c1
DNA Barcode:
CCTCTCAAGTGCTGGGATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nop16 (28126)
Gene Description:
NOP16 nucleolar protein
Transcript:
RefSeq NM_178605.2 (NON-CURRENT)
Match location:
Position 913 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190964)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTCAAGTGCTGGGATTAA
Hairpin Sequence:
5'-CCGG-CCTCTCAAGTGCTGGGATTAA-CTCGAG-TTAATCCCAGCACTTGAGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTCAAGTGCTGGGATTAACTCGAGTTAATCCCAGCACTTGAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTCAAGTGCTGGGATTAACTCGAGTTAATCCCAGCACTTGAGAGG-3'

Other clones with same target sequence:

(none)