Construct: shRNA TRCN0000190996

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001008973.1-893s1c1
DNA Barcode:
CTACTACAAGTATCCGAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem232 (381107)
Gene Description:
transmembrane protein 232
Transcript:
RefSeq NM_001008973.1 (NON-CURRENT)
Match location:
Position 893 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190996)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACTACAAGTATCCGAACAT
Hairpin Sequence:
5'-CCGG-CTACTACAAGTATCCGAACAT-CTCGAG-ATGTTCGGATACTTGTAGTAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACTACAAGTATCCGAACATCTCGAGATGTTCGGATACTTGTAGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACTACAAGTATCCGAACATCTCGAGATGTTCGGATACTTGTAGTAG-3'

Other clones with same target sequence:

(none)