Construct: shRNA TRCN0000190999

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025423.1-912s1c1
DNA Barcode:
CGTACAATCATGTGTAATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1110059E24Rik (66206)
Gene Description:
RIKEN cDNA 1110059E24 gene
Transcript:
RefSeq NM_025423.1 (NON-CURRENT)
Match location:
Position 912 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190999)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTACAATCATGTGTAATGAT
Hairpin Sequence:
5'-CCGG-CGTACAATCATGTGTAATGAT-CTCGAG-ATCATTACACATGATTGTACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTACAATCATGTGTAATGATCTCGAGATCATTACACATGATTGTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTACAATCATGTGTAATGATCTCGAGATCATTACACATGATTGTACG-3'

Other clones with same target sequence:

(none)