Construct: shRNA TRCN0000191003

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027583.1-770s1c1
DNA Barcode:
CGCAAGTTTCATTGAGACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spata16 (70862)
Gene Description:
spermatogenesis associated 16
Transcript:
RefSeq NM_027583.1 (NON-CURRENT)
Match location:
Position 770 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191003)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAAGTTTCATTGAGACAAA
Hairpin Sequence:
5'-CCGG-CGCAAGTTTCATTGAGACAAA-CTCGAG-TTTGTCTCAATGAAACTTGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAAGTTTCATTGAGACAAACTCGAGTTTGTCTCAATGAAACTTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAAGTTTCATTGAGACAAACTCGAGTTTGTCTCAATGAAACTTGCG-3'

Other clones with same target sequence:

(none)