Construct: shRNA TRCN0000191015

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001029930.1-258s1c1
DNA Barcode:
CTAGAAGAACTGAAATTGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm5796 (545007)
Gene Description:
predicted gene 5796
Transcript:
RefSeq NM_001029930.1 (NON-CURRENT)
Match location:
Position 258 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191015)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGAAGAACTGAAATTGGAT
Hairpin Sequence:
5'-CCGG-CTAGAAGAACTGAAATTGGAT-CTCGAG-ATCCAATTTCAGTTCTTCTAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGAAGAACTGAAATTGGATCTCGAGATCCAATTTCAGTTCTTCTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGAAGAACTGAAATTGGATCTCGAGATCCAATTTCAGTTCTTCTAG-3'

Other clones with same target sequence:

(none)