Construct: shRNA TRCN0000191029

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172456.1-586s1c1
DNA Barcode:
CGGAGAGATTTGAAGATGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Exog (208194)
Gene Description:
endo/exonuclease (5'-3'), endonuclease G-like
Transcript:
RefSeq NM_172456.1 (NON-CURRENT)
Match location:
Position 586 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191029)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGAGATTTGAAGATGTTT
Hairpin Sequence:
5'-CCGG-CGGAGAGATTTGAAGATGTTT-CTCGAG-AAACATCTTCAAATCTCTCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGAGATTTGAAGATGTTTCTCGAGAAACATCTTCAAATCTCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGAGATTTGAAGATGTTTCTCGAGAAACATCTTCAAATCTCTCCG-3'

Other clones with same target sequence:

(none)