Construct: shRNA TRCN0000191059
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_175254.2-290s1c1
- DNA Barcode:
- CGCTGTGTTTAAGAAATCAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- 9330180L21Rik (77268)
- Gene Description:
- RIKEN cDNA 9330180L21 gene
- Transcript:
- RefSeq NM_175254.2 (NON-CURRENT)
- Match location:
- Position 290 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000191059)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGCTGTGTTTAAGAAATCAAA
- Hairpin Sequence:
-
5'-CCGG-CGCTGTGTTTAAGAAATCAAA-CTCGAG-TTTGATTTCTTAAACACAGCG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGCTGTGTTTAAGAAATCAAACTCGAGTTTGATTTCTTAAACACAGCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGCTGTGTTTAAGAAATCAAACTCGAGTTTGATTTCTTAAACACAGCG-3'
Other clones with same target sequence:
(none)
