Construct: shRNA TRCN0000191104

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025719.1-840s1c1
DNA Barcode:
GCAGAGAGCATGGATTTAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nkapl (66707)
Gene Description:
NFKB activating protein-like
Transcript:
RefSeq NM_025719.1 (NON-CURRENT)
Match location:
Position 840 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191104)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGAGCATGGATTTAATA
Hairpin Sequence:
5'-CCGG-GCAGAGAGCATGGATTTAATA-CTCGAG-TATTAAATCCATGCTCTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGAGCATGGATTTAATACTCGAGTATTAAATCCATGCTCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGAGCATGGATTTAATACTCGAGTATTAAATCCATGCTCTCTGC-3'

Other clones with same target sequence:

(none)