Construct: shRNA TRCN0000191116

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177585.2-1317s1c1
DNA Barcode:
GCTTGGCATTATTGCCTAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Iqcj (208426)
Gene Description:
IQ motif containing J
Transcript:
RefSeq NM_177585.2 (NON-CURRENT)
Match location:
Position 1317 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191116)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGGCATTATTGCCTAATA
Hairpin Sequence:
5'-CCGG-GCTTGGCATTATTGCCTAATA-CTCGAG-TATTAGGCAATAATGCCAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGGCATTATTGCCTAATACTCGAGTATTAGGCAATAATGCCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGGCATTATTGCCTAATACTCGAGTATTAGGCAATAATGCCAAGC-3'

Other clones with same target sequence:

(none)