Construct: shRNA TRCN0000191126

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019914.2-1384s1c1
DNA Barcode:
CGGAGCTGTTTATTCATTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mllt11 (56772)
Gene Description:
myeloid/lymphoid or mixed-lineage leukemia; translocated to, 11
Transcript:
RefSeq NM_019914.2 (NON-CURRENT)
Match location:
Position 1384 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191126)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGCTGTTTATTCATTTAT
Hairpin Sequence:
5'-CCGG-CGGAGCTGTTTATTCATTTAT-CTCGAG-ATAAATGAATAAACAGCTCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGCTGTTTATTCATTTATCTCGAGATAAATGAATAAACAGCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGCTGTTTATTCATTTATCTCGAGATAAATGAATAAACAGCTCCG-3'

Other clones with same target sequence:

(none)