Construct: shRNA TRCN0000191129

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025714.1-439s1c1
DNA Barcode:
CGTGTGAAATTAGCCTTAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Odf2l (52184)
Gene Description:
outer dense fiber of sperm tails 2-like
Transcript:
RefSeq NM_025714.1 (NON-CURRENT)
Match location:
Position 439 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191129)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGTGAAATTAGCCTTAGAA
Hairpin Sequence:
5'-CCGG-CGTGTGAAATTAGCCTTAGAA-CTCGAG-TTCTAAGGCTAATTTCACACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGTGAAATTAGCCTTAGAACTCGAGTTCTAAGGCTAATTTCACACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGTGAAATTAGCCTTAGAACTCGAGTTCTAAGGCTAATTTCACACG-3'

Other clones with same target sequence:

(none)