Construct: shRNA TRCN0000191196

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177670.2-1059s1c1
DNA Barcode:
CCTTGATGATAATGAACTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem69 (230657)
Gene Description:
transmembrane protein 69
Transcript:
RefSeq NM_177670.2 (NON-CURRENT)
Match location:
Position 1059 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191196)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGATGATAATGAACTGAA
Hairpin Sequence:
5'-CCGG-CCTTGATGATAATGAACTGAA-CTCGAG-TTCAGTTCATTATCATCAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGATGATAATGAACTGAACTCGAGTTCAGTTCATTATCATCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGATGATAATGAACTGAACTCGAGTTCAGTTCATTATCATCAAGG-3'

Other clones with same target sequence:

(none)