Construct: shRNA TRCN0000191202

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198118.1-3425s1c1
DNA Barcode:
CCTTGATTCATTCAGTCTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
9130019P16Rik (378878)
Gene Description:
RIKEN cDNA 9130019P16 gene
Transcript:
RefSeq NM_198118.1 (NON-CURRENT)
Match location:
Position 3425 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191202)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGATTCATTCAGTCTTAA
Hairpin Sequence:
5'-CCGG-CCTTGATTCATTCAGTCTTAA-CTCGAG-TTAAGACTGAATGAATCAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGATTCATTCAGTCTTAACTCGAGTTAAGACTGAATGAATCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGATTCATTCAGTCTTAACTCGAGTTAAGACTGAATGAATCAAGG-3'

Other clones with same target sequence:

(none)