Construct: shRNA TRCN0000191234
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_175666.1-747s1c1
- DNA Barcode:
- CGACTTAAAGTGAGATGGTTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Hist2h2bb (319189)
- Gene Description:
- histone cluster 2, H2bb
- Transcript:
- RefSeq NM_175666.1 (NON-CURRENT)
- Match location:
- Position 747 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000191234)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGACTTAAAGTGAGATGGTTT
- Hairpin Sequence:
-
5'-CCGG-CGACTTAAAGTGAGATGGTTT-CTCGAG-AAACCATCTCACTTTAAGTCG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGACTTAAAGTGAGATGGTTTCTCGAGAAACCATCTCACTTTAAGTCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGACTTAAAGTGAGATGGTTTCTCGAGAAACCATCTCACTTTAAGTCG-3'
Other clones with same target sequence:
(none)
