Construct: shRNA TRCN0000191317

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144906.1-3563s1c1
DNA Barcode:
CCTTGTTTCTTCTTTACTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sgip1 (73094)
Gene Description:
SH3-domain GRB2-like (endophilin) interacting protein 1
Transcript:
RefSeq NM_144906.1 (NON-CURRENT)
Match location:
Position 3563 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191317)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGTTTCTTCTTTACTCAT
Hairpin Sequence:
5'-CCGG-CCTTGTTTCTTCTTTACTCAT-CTCGAG-ATGAGTAAAGAAGAAACAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGTTTCTTCTTTACTCATCTCGAGATGAGTAAAGAAGAAACAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGTTTCTTCTTTACTCATCTCGAGATGAGTAAAGAAGAAACAAGG-3'

Other clones with same target sequence:

(none)