Construct: shRNA TRCN0000191327

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178670.2-595s1c1
DNA Barcode:
CTGATTCAGATACATTGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
8030462N17Rik (212163)
Gene Description:
RIKEN cDNA 8030462N17 gene
Transcript:
RefSeq NM_178670.2 (NON-CURRENT)
Match location:
Position 595 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191327)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGATTCAGATACATTGTCTT
Hairpin Sequence:
5'-CCGG-CTGATTCAGATACATTGTCTT-CTCGAG-AAGACAATGTATCTGAATCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGATTCAGATACATTGTCTTCTCGAGAAGACAATGTATCTGAATCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGATTCAGATACATTGTCTTCTCGAGAAGACAATGTATCTGAATCAG-3'

Other clones with same target sequence:

(none)