Construct: shRNA TRCN0000191348

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172282.1-2410s1c1
DNA Barcode:
CCAGAGTAATTTATTTGCAGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmco3 (234076)
Gene Description:
transmembrane and coiled-coil domains 3
Transcript:
RefSeq NM_172282.1 (NON-CURRENT)
Match location:
Position 2410 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191348)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGTAATTTATTTGCAGT
Hairpin Sequence:
5'-CCGG-CCAGAGTAATTTATTTGCAGT-CTCGAG-ACTGCAAATAAATTACTCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGTAATTTATTTGCAGTCTCGAGACTGCAAATAAATTACTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGTAATTTATTTGCAGTCTCGAGACTGCAAATAAATTACTCTGG-3'

Other clones with same target sequence:

  1. TRCN0000314392