Construct: shRNA TRCN0000191435

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026126.2-1261s1c1
DNA Barcode:
CCAGGCATTCAGAATAAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fundc2 (67391)
Gene Description:
FUN14 domain containing 2
Transcript:
RefSeq NM_026126.2 (NON-CURRENT)
Match location:
Position 1261 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191435)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGCATTCAGAATAAGAAT
Hairpin Sequence:
5'-CCGG-CCAGGCATTCAGAATAAGAAT-CTCGAG-ATTCTTATTCTGAATGCCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGCATTCAGAATAAGAATCTCGAGATTCTTATTCTGAATGCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGCATTCAGAATAAGAATCTCGAGATTCTTATTCTGAATGCCTGG-3'

Other clones with same target sequence:

(none)