Construct: shRNA TRCN0000191453

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027420.2-522s1c1
DNA Barcode:
CTTCATAGATTCCTTAGCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arpin (70420)
Gene Description:
actin-related protein 2/3 complex inhibitor
Transcript:
RefSeq NM_027420.2 (NON-CURRENT)
Match location:
Position 522 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191453)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCATAGATTCCTTAGCCAA
Hairpin Sequence:
5'-CCGG-CTTCATAGATTCCTTAGCCAA-CTCGAG-TTGGCTAAGGAATCTATGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCATAGATTCCTTAGCCAACTCGAGTTGGCTAAGGAATCTATGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCATAGATTCCTTAGCCAACTCGAGTTGGCTAAGGAATCTATGAAG-3'

Other clones with same target sequence:

(none)