Construct: shRNA TRCN0000191596

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172677.2-369s1c1
DNA Barcode:
GCCAGACAAATCAGAAATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ythdf3 (229096)
Gene Description:
YTH domain family 3
Transcript:
RefSeq NM_172677.2 (NON-CURRENT)
Match location:
Position 369 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191596)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGACAAATCAGAAATATA
Hairpin Sequence:
5'-CCGG-GCCAGACAAATCAGAAATATA-CTCGAG-TATATTTCTGATTTGTCTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGACAAATCAGAAATATACTCGAGTATATTTCTGATTTGTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGACAAATCAGAAATATACTCGAGTATATTTCTGATTTGTCTGGC-3'

Other clones with same target sequence:

(none)