Construct: shRNA TRCN0000191626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032543.1-168s1c1
DNA Barcode:
GAAACCGCTAAACTTCCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf123 (84585)
Gene Description:
ring finger protein 123
Transcript:
RefSeq NM_032543.1 (NON-CURRENT)
Match location:
Position 168 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAACCGCTAAACTTCCATAA
Hairpin Sequence:
5'-CCGG-GAAACCGCTAAACTTCCATAA-CTCGAG-TTATGGAAGTTTAGCGGTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAACCGCTAAACTTCCATAACTCGAGTTATGGAAGTTTAGCGGTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAACCGCTAAACTTCCATAACTCGAGTTATGGAAGTTTAGCGGTTTC-3'

Other clones with same target sequence:

  1. TRCN0000346890