Construct: shRNA TRCN0000191646

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080858.1-660s1c1
DNA Barcode:
GAGGCTAATGTCAAAGCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Asb12 (70392)
Gene Description:
ankyrin repeat and SOCS box-containing 12
Transcript:
RefSeq NM_080858.1 (NON-CURRENT)
Match location:
Position 660 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191646)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGCTAATGTCAAAGCTAAA
Hairpin Sequence:
5'-CCGG-GAGGCTAATGTCAAAGCTAAA-CTCGAG-TTTAGCTTTGACATTAGCCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGCTAATGTCAAAGCTAAACTCGAGTTTAGCTTTGACATTAGCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGCTAATGTCAAAGCTAAACTCGAGTTTAGCTTTGACATTAGCCTC-3'

Other clones with same target sequence:

(none)