Construct: shRNA TRCN0000191770

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138587.3-171s1c1
DNA Barcode:
GTATTCTTACTGACCTTCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam3c (27999)
Gene Description:
family with sequence similarity 3, member C
Transcript:
RefSeq NM_138587.3 (NON-CURRENT)
Match location:
Position 171 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191770)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATTCTTACTGACCTTCTAT
Hairpin Sequence:
5'-CCGG-GTATTCTTACTGACCTTCTAT-CTCGAG-ATAGAAGGTCAGTAAGAATAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATTCTTACTGACCTTCTATCTCGAGATAGAAGGTCAGTAAGAATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATTCTTACTGACCTTCTATCTCGAGATAGAAGGTCAGTAAGAATAC-3'

Other clones with same target sequence:

  1. TRCN0000292739