Construct: shRNA TRCN0000191776

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178673.3-3446s1c1
DNA Barcode:
GCAATGAATCTACTGTCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fstl5 (213262)
Gene Description:
follistatin-like 5
Transcript:
RefSeq NM_178673.3 (NON-CURRENT)
Match location:
Position 3446 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191776)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATGAATCTACTGTCATTA
Hairpin Sequence:
5'-CCGG-GCAATGAATCTACTGTCATTA-CTCGAG-TAATGACAGTAGATTCATTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATGAATCTACTGTCATTACTCGAGTAATGACAGTAGATTCATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATGAATCTACTGTCATTACTCGAGTAATGACAGTAGATTCATTGC-3'

Other clones with same target sequence:

(none)