Construct: shRNA TRCN0000191799

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145568.3-1246s1c1
DNA Barcode:
GCATTCATATTCATGTCACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Krcc1 (57896)
Gene Description:
lysine-rich coiled-coil 1
Transcript:
RefSeq NM_145568.3 (CURRENT)
Match location:
Position 1246 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191799)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTCATATTCATGTCACTT
Hairpin Sequence:
5'-CCGG-GCATTCATATTCATGTCACTT-CTCGAG-AAGTGACATGAATATGAATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTCATATTCATGTCACTTCTCGAGAAGTGACATGAATATGAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTCATATTCATGTCACTTCTCGAGAAGTGACATGAATATGAATGC-3'

Other clones with same target sequence:

(none)