Construct: shRNA TRCN0000191812

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033350.1-782s1c1
DNA Barcode:
GCGAAATTCTAGAGGTTGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bank1 (242248)
Gene Description:
B cell scaffold protein with ankyrin repeats 1
Transcript:
RefSeq NM_001033350.1 (NON-CURRENT)
Match location:
Position 782 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191812)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAAATTCTAGAGGTTGAAT
Hairpin Sequence:
5'-CCGG-GCGAAATTCTAGAGGTTGAAT-CTCGAG-ATTCAACCTCTAGAATTTCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAAATTCTAGAGGTTGAATCTCGAGATTCAACCTCTAGAATTTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAAATTCTAGAGGTTGAATCTCGAGATTCAACCTCTAGAATTTCGC-3'

Other clones with same target sequence:

(none)