Construct: shRNA TRCN0000191850
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_029035.2-1349s1c1
- DNA Barcode:
- GCAGAGAATAAACTATGAGAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Spsb1 (74646)
- Gene Description:
- splA/ryanodine receptor domain and SOCS box containing 1
- Transcript:
- RefSeq NM_029035.2 (CURRENT)
- Match location:
- Position 1349 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000191850)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAGAGAATAAACTATGAGAA
- Hairpin Sequence:
-
5'-CCGG-GCAGAGAATAAACTATGAGAA-CTCGAG-TTCTCATAGTTTATTCTCTGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAGAGAATAAACTATGAGAACTCGAGTTCTCATAGTTTATTCTCTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAGAGAATAAACTATGAGAACTCGAGTTCTCATAGTTTATTCTCTGC-3'
Other clones with same target sequence:
(none)
