Construct: shRNA TRCN0000191853

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030172.1-1913s1c1
DNA Barcode:
GCAGAAATGAATTACTGGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Efcab11 (78767)
Gene Description:
EF-hand calcium binding domain 11
Transcript:
RefSeq NM_030172.1 (NON-CURRENT)
Match location:
Position 1913 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000191853)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAATGAATTACTGGTAT
Hairpin Sequence:
5'-CCGG-GCAGAAATGAATTACTGGTAT-CTCGAG-ATACCAGTAATTCATTTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAATGAATTACTGGTATCTCGAGATACCAGTAATTCATTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAATGAATTACTGGTATCTCGAGATACCAGTAATTCATTTCTGC-3'

Other clones with same target sequence:

(none)