Construct: shRNA TRCN0000192009

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026242.1-1084s1c1
DNA Barcode:
CGGAGGATAGTATTGTTTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mrfap1 (67568)
Gene Description:
Morf4 family associated protein 1
Transcript:
RefSeq NM_026242.1 (NON-CURRENT)
Match location:
Position 1084 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192009)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGGATAGTATTGTTTCTA
Hairpin Sequence:
5'-CCGG-CGGAGGATAGTATTGTTTCTA-CTCGAG-TAGAAACAATACTATCCTCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGGATAGTATTGTTTCTACTCGAGTAGAAACAATACTATCCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGGATAGTATTGTTTCTACTCGAGTAGAAACAATACTATCCTCCG-3'

Other clones with same target sequence:

(none)