Construct: shRNA TRCN0000192094

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023605.1-1059s1c1
DNA Barcode:
CTGGAGAGAGATGTTTCTAGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxo9 (71538)
Gene Description:
f-box protein 9
Transcript:
RefSeq NM_023605.1 (NON-CURRENT)
Match location:
Position 1059 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192094)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGAGAGAGATGTTTCTAGA
Hairpin Sequence:
5'-CCGG-CTGGAGAGAGATGTTTCTAGA-CTCGAG-TCTAGAAACATCTCTCTCCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGAGAGAGATGTTTCTAGACTCGAGTCTAGAAACATCTCTCTCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGAGAGAGATGTTTCTAGACTCGAGTCTAGAAACATCTCTCTCCAG-3'

Other clones with same target sequence:

  1. TRCN0000279134