Construct: shRNA TRCN0000192133

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001034908.1-832s1c1
DNA Barcode:
GAGCAGAATTACACATGCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC547343 (547343)
Gene Description:
similar to H-2 class I histocompatibility antigen, L-D alpha chain precursor
Transcript:
RefSeq NM_001034908.1 (NON-CURRENT)
Match location:
Position 832 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192133)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCAGAATTACACATGCCAT
Hairpin Sequence:
5'-CCGG-GAGCAGAATTACACATGCCAT-CTCGAG-ATGGCATGTGTAATTCTGCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCAGAATTACACATGCCATCTCGAGATGGCATGTGTAATTCTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCAGAATTACACATGCCATCTCGAGATGGCATGTGTAATTCTGCTC-3'

Other clones with same target sequence:

  1. TRCN0000066903