Construct: shRNA TRCN0000192141

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026681.3-435s1c1
DNA Barcode:
GCAGCTGATCGTAATGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc88c (68339)
Gene Description:
coiled-coil domain containing 88C
Transcript:
RefSeq NM_026681.3 (NON-CURRENT)
Match location:
Position 435 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192141)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCTGATCGTAATGAATTT
Hairpin Sequence:
5'-CCGG-GCAGCTGATCGTAATGAATTT-CTCGAG-AAATTCATTACGATCAGCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCTGATCGTAATGAATTTCTCGAGAAATTCATTACGATCAGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCTGATCGTAATGAATTTCTCGAGAAATTCATTACGATCAGCTGC-3'

Other clones with same target sequence:

(none)