Construct: shRNA TRCN0000192142
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_027478.1-829s1c1
- DNA Barcode:
- GCATCATGTAAGAGTTGTGAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Tmem230 (70612)
- Gene Description:
- transmembrane protein 230
- Transcript:
- RefSeq NM_027478.1 (NON-CURRENT)
- Match location:
- Position 829 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000192142)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCATCATGTAAGAGTTGTGAA
- Hairpin Sequence:
-
5'-CCGG-GCATCATGTAAGAGTTGTGAA-CTCGAG-TTCACAACTCTTACATGATGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCATCATGTAAGAGTTGTGAACTCGAGTTCACAACTCTTACATGATGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCATCATGTAAGAGTTGTGAACTCGAGTTCACAACTCTTACATGATGC-3'
Other clones with same target sequence:
-
TRCN0000320170
