Construct: shRNA TRCN0000192162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001025572.1-7265s1c1
DNA Barcode:
CCCAAACAACAACAACAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ankrd12 (106585)
Gene Description:
ankyrin repeat domain 12
Transcript:
RefSeq NM_001025572.1 (CURRENT)
Match location:
Position 7265 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAACAACAACAACAACAA
Hairpin Sequence:
5'-CCGG-CCCAAACAACAACAACAACAA-CTCGAG-TTGTTGTTGTTGTTGTTTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAACAACAACAACAACAACTCGAGTTGTTGTTGTTGTTGTTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAACAACAACAACAACAACTCGAGTTGTTGTTGTTGTTGTTTGGG-3'

Other clones with same target sequence:

(none)