Construct: shRNA TRCN0000192244

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001025241.1-829s1c1
DNA Barcode:
CTTCTTAATAGGTTCCTGGGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm20747 (434960)
Gene Description:
predicted gene, 20747
Transcript:
RefSeq NM_001025241.1 (NON-CURRENT)
Match location:
Position 829 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192244)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTTAATAGGTTCCTGGGT
Hairpin Sequence:
5'-CCGG-CTTCTTAATAGGTTCCTGGGT-CTCGAG-ACCCAGGAACCTATTAAGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTTAATAGGTTCCTGGGTCTCGAGACCCAGGAACCTATTAAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTTAATAGGTTCCTGGGTCTCGAGACCCAGGAACCTATTAAGAAG-3'

Other clones with same target sequence:

(none)