Construct: shRNA TRCN0000192245

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001025380.1-400s1c1
DNA Barcode:
CCAGAGTCTATGGTTGCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adam39 (546055)
Gene Description:
a disintegrin and metallopeptidase domain 39
Transcript:
RefSeq NM_001025380.1 (NON-CURRENT)
Match location:
Position 400 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192245)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGTCTATGGTTGCTATT
Hairpin Sequence:
5'-CCGG-CCAGAGTCTATGGTTGCTATT-CTCGAG-AATAGCAACCATAGACTCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGTCTATGGTTGCTATTCTCGAGAATAGCAACCATAGACTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGTCTATGGTTGCTATTCTCGAGAATAGCAACCATAGACTCTGG-3'

Other clones with same target sequence:

  1. TRCN0000032157