Construct: shRNA TRCN0000192339

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025829.2-202s1c1
DNA Barcode:
CGGTACAAACAGTGCAGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif4e3 (66892)
Gene Description:
eukaryotic translation initiation factor 4E member 3
Transcript:
RefSeq NM_025829.2 (NON-CURRENT)
Match location:
Position 202 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192339)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTACAAACAGTGCAGATAT
Hairpin Sequence:
5'-CCGG-CGGTACAAACAGTGCAGATAT-CTCGAG-ATATCTGCACTGTTTGTACCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTACAAACAGTGCAGATATCTCGAGATATCTGCACTGTTTGTACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTACAAACAGTGCAGATATCTCGAGATATCTGCACTGTTTGTACCG-3'

Other clones with same target sequence:

  1. TRCN0000292916