Construct: shRNA TRCN0000192357

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176932.2-2208s1c1
DNA Barcode:
CTCTGGACAAGGATTAACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
6430510M02Rik (319517)
Gene Description:
RIKEN cDNA 6430510M02 gene
Transcript:
RefSeq NM_176932.2 (NON-CURRENT)
Match location:
Position 2208 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192357)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTGGACAAGGATTAACTTT
Hairpin Sequence:
5'-CCGG-CTCTGGACAAGGATTAACTTT-CTCGAG-AAAGTTAATCCTTGTCCAGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTGGACAAGGATTAACTTTCTCGAGAAAGTTAATCCTTGTCCAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTGGACAAGGATTAACTTTCTCGAGAAAGTTAATCCTTGTCCAGAG-3'

Other clones with same target sequence:

(none)