Construct: shRNA TRCN0000192431

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027898.2-192s1c1
DNA Barcode:
CGGAAACTGTTCAGCAAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gramd1a (52857)
Gene Description:
GRAM domain containing 1A
Transcript:
RefSeq NM_027898.2 (NON-CURRENT)
Match location:
Position 192 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192431)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAAACTGTTCAGCAAACTT
Hairpin Sequence:
5'-CCGG-CGGAAACTGTTCAGCAAACTT-CTCGAG-AAGTTTGCTGAACAGTTTCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAAACTGTTCAGCAAACTTCTCGAGAAGTTTGCTGAACAGTTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAAACTGTTCAGCAAACTTCTCGAGAAGTTTGCTGAACAGTTTCCG-3'

Other clones with same target sequence:

(none)