Construct: shRNA TRCN0000192449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024706.1-599s1c1
DNA Barcode:
CAGATGGATCTCTGCATTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm5458 (432825)
Gene Description:
predicted gene 5458
Transcript:
RefSeq NM_001024706.1 (NON-CURRENT)
Match location:
Position 599 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATGGATCTCTGCATTCTT
Hairpin Sequence:
5'-CCGG-CAGATGGATCTCTGCATTCTT-CTCGAG-AAGAATGCAGAGATCCATCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATGGATCTCTGCATTCTTCTCGAGAAGAATGCAGAGATCCATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATGGATCTCTGCATTCTTCTCGAGAAGAATGCAGAGATCCATCTG-3'

Other clones with same target sequence:

(none)